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Comprehensive toxicology evaluations reveal that the compound does not affect food intake, body temperature regulation, or basic physiological parameters at effective doses in animal models, indicating that its metabolic effects occur through specific pathway modulation rather than general physiological disruption

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At the same time, levels of the cyclin-dependent kinase inhibitor, p21, were increased in the presence of DIM, resulting in G1-phase cell cycle arrest [121, 122]

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